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KMID : 0385219960060030054
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Korean Journal of Gerontology
1996 Volume.6 No. 3 p.54 ~ p.61
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The Effect of Age and Dietary Fat level on Glucose Transport and Immune Response in Rats
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Kim Hwa-Young
Park Yi-Kyung
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Abstract
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The effect of dietary fat level on glucose transport and immune response were investigated in Sprague-Dawley male rats of different age group ; rats of 2 months and 12 months of age were fed either 20%(w/w) or 6% fat diet for 21 days. Glucose transport in adipocyte and splenocyte mitogenesis were measured, along with lipids concentrations in serum and liver. Total lipid, triglyceride and cholesterol concentrations in serum were not affected by age and dietary fat level. the contents of total lipid, cholesterol and triglyceride in liver were significantly higher in high fat diet fed rats than in those of low fat diet, however, age had no effect on liver lipids. Glucose transport was assessed in isolated adipocytes from epididymal fat pad. Glucose transport into adipocytes was significantly decreased in old rats compared to young rats. The two age group showed different responses of dietary fat level on glucose transport into adipocytes. In old rats it was lower in high fat diet group, on the other hand, young rats fed high fat diet showed a tendency of higher glucose transport compared to young rats in low fat diet group. The weights of lymphoid organ, the spleen and thymus, were significantly lower in old rats. To assess immune function, mitogenesis of spleen cell were measured using con A, PHA and PWM as mitogens. There was no difference in motogenesis between 2 age groups. Rats in high fat diet group showed lower cell proliferation response by Con A and PHA, but not by PWM. In conclusion, fat accumulation assessed by epididymal fat pad weight was increased and glucose transport was decreased in rats of 12 months of age compared to 2 months old rats. This tendency was more obvious when old rats were fed high fat diet. There was no significant effect of age on splenocyte motogenesis, however, increasing dietary fat seems to show lower response of proliferation by Con A and PHA. Therefore, high fat diet may have an effect of lowering glucose transport and immune function in old rats.
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KEYWORD
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age, fat level, glucose transport, immune response mitogenesis
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